Janecka, J.E., Jackson, R., Yuquang, Z., Diqiang, L., Munkhtsog, B., et al. (2008). Population monitoring of snow leopards using noninvasive collection of scat samples: a pilot study (Vol. 11).
Abstract: The endangered snow leopard Panthera uncia occurs in rugged, high-altitude regions of Central Asia. However, information on the status of this felid is limited in many areas. We conducted a pilot study to optimize molecular markers for the analysis of snow leopard scat samples and to examine the feasibility of using noninvasive genetic methods for monitoring this felid. We designed snow leopard-specific primers for seven microsatellite loci that amplified shorter segments and avoided flanking sequences shared with repetitive elements. By redesigning primers we maximized genotyping success and minimized genotyping errors. In addition, we tested a Y chromosome-marker for sex identification and designed a panel of mitochondrial DNA primers for examining genetic diversity of snow leopards using scat samples. We collected scats believed to be from snow leopards in three separate geographic regions including north-western India, central China and southern Mongolia. We observed snow leopard scats in all three sites despite only brief 2-day surveys in each area. There was a high rate of species misidentification in the field with up to 54% of snow leopard scats misidentified as red fox. The high rate of field misidentification suggests sign surveys incorporating scat likely overestimate snow leopard abundance. The highest ratio of snow leopard scats was observed in Ladakh (India) and South Gobi (Mongolia), where four and five snow leopards were detected, respectively. Our findings describe a species-specific molecular panel for analysis of snow leopard scats, and highlight the efficacy of noninvasive genetic surveys for monitoring snow leopards. These methods enable large-scale noninvasive studies that will provide information critical for conservation of snow leopards.
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Janeč, & ka, J. E., Munkhtsog, B., Jackson, R.M., Naranbaatar, G., Mallon, D.P. & Murphy, W.J. (2011). Comparison of noninvasive genetic and camera-trapping techniques for surveying snow leopards. Journal of Mammalogy, 92(4), 771–783.
Abstract: The endangered snow leopard (Panthera uncia) is widely but sparsely distributed throughout the mountainous regions of central Asia. Detailed information on the status and abundance of the snow leopard is limited because of the logistical challenges faced when working in the rugged terrain it occupies, along with its secretive nature. Camera-trapping and noninvasive genetic techniques have been used successfully to survey this felid. We compared noninvasive genetic and camera-trapping snow leopard surveys in the Gobi Desert of Mongolia. We collected 180 putative snow leopard scats from 3 sites during an 8-day period along 37.74 km of transects. We then conducted a 65-day photographic survey at 1 of these sites, approximately 2 months after scat collection. In the site where both techniques were used noninvasive genetics detected 5 individuals in only 2 days of fieldwork compared to 7 individuals observed in the 65-day camera-trapping session. Estimates of population size from noninvasive genetics ranged between 16 and 19 snow leopards in the 314.3-km2 area surveyed, yielding densities of 4.9–5.9 individuals/100 km2. In comparison, the population estimate from the 65-day photographic survey was 4 individuals (adults only) within the 264-km2 area, for a density estimate of 1.5 snow leopards/100 km2. Higher density estimates from the noninvasive genetic survey were due partly to an inability to determine age and exclude subadults, reduced spatial distribution of sampling points as a consequence of collecting scats along linear transects, and deposition of scats by multiple snow leopards on common sites. Resulting differences could inflate abundance estimated from noninvasive genetic surveys and prevent direct comparison of densities derived from the 2 approaches unless appropriate adjustments are made to the study design.
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Jackson, R. M., Ahlborn, G., Gurung, M., & Ale, S. (1996). Reducing livestock depredation losses in the Nepalese Himalaya. Proc.Vertebr.Pest Conf, 17, 241–247.
Abstract: The authors investigated livestock depredation patterns of snow leopards on the northern slopes of the Himalayans near the villages of Manang and Khangshar, Nepal. Information is discussed on the relationships among livestock loss, endangered species management, public relations/conservation issues, and cooperative efforts among institutions involved in the decision making process. A plan is devised for alleviating livestock loss and protecting endangered species in the area. pcp
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Jackson, R. (1990). Snow Leopard Recovery Plan: Preliminary Recommendations.
Abstract: Submitted to Species Survival Commission, Cat Specialist Group 31 January 1990.
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Jackson, R., Roe, J., Wangchuk, R., & Hunter, D. (2005). Camera-Trapping of Snow Leopards. Cat News, 42(Spring), 19–21.
Abstract: Solitary felids like tigers and snow leopards are notoriously difficult to enumerate, and indirect techniques like pugmark surveys often produce ambiguous information that is difficult to interpret because many factors influence marking behavior and frequency (Ahlborn & Jackson 1988). Considering the snow leopard's rugged habitat, it is not surprising then that information on its current status and occupied range is very limited. We adapted the camera-trapping techniques pioneered by Ullas Karanth and his associates for counting Bengal tigers to the census taking of snow leopards in the Rumbak watershed of the India's Hemis High Altitude National Park (HNP), located in Ladakh near Leh (76ø 50' to 77ø 45' East; 33ø 15' to 34ø 20'North).
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Ale, S., Thapa, K., Jackson, R., Smith, J.L.D. (2010). The fate of snow leopards in and around Mt. Everest. Cat News, 53(Autumn), 19–21.
Abstract: Since the early 2000s snow leopards Panthera uncia have re-colonized the southern slopes of Mt. Everest after several decades of extirpation. Are they now beginning to disperse to the adjoining valleys that may serve as habitat corridors linking the Everest region to other protected areas in Nepal? We conducted a cursory survey in autumn 2009 in Rolwaling lying west of Mt. Everest and detected snow leopard presence. We conclude that in these remote valleys snow leopards must rely upon livestock given the low abundance of natural prey, Himalayan tahr. Livestock-rearing is unfortunately declining in the region. Rolwaling requires immediate conservation attention for the continued survival of the endangered snow leopard and other high altitude flora and fauna.
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Jackson, R. M. (1979). Snow Leopards in Nepal. Oryx, 15, 191–195.
Abstract: Reviews in detail occurence, status, and conservation measures related to snow leopards in Nepal. Estimates 150-300 snow leopards in Nepal. Local hunters can get 10 to 50 US dollars for a pelt
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Jackson, R., Ahlborn, G.G. (1986). Appendix: Snow leopard managment recommendations provided to HMG in: Himalayan Snow Leopard Project: Final Progress Report, Phase I. Report: 1-7. Himalayan.
Abstract: Preliminary recommendations for the management of snow leopard and its prey are provided for the Langu Valley segment of the Shey-Pkoksundo National Park. Park-wide and country-wide conservation options and management recommendations await results of the surveys scheduled for 1987. The following management objectives are formulated: 1) Protection and ultimate restoration of all natural communities within the area 2) Special protection measures for snow leopard and musk deer (strict control of hunting and livestock grazing) 3) Secure natural resources around local villages 4) Respect traditional rights of villagers, while controlling high impact human activities 5) Secure cooperation of local people. These objectives are refined and recommendations for concrete conservation actions are made.
Notes: document is a part of the Himalayan Snow Leopard Project: Final Progress Report, Phase I
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Jackson, R., & Fox, J. L. (2000). Report on Fifth Slims Training Workshop (Nepal) (Vol. xvii). Seattle: International Snow Leopard Trust.
Abstract: Nepal's snow leopards (Uncia uncia) are mostly found along the northern border with Tibet (China). The largest populations are in Dolpa, Mugu, Manang, and Myagdi Districts. Potential habitat totals about 30,000 square kilometers. Numbers are estimated at 300-500, but surveys are urgently needed to confirm this rough guess. Like elsewhere, the primary threats center on poaching, depletion of natural prey, livestock depredation and resultant retributive killing of snow leopards by herders, and the lack of public awareness and support for conserving snow leoaprds, especially among local herders.
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Janecka, J. E., Jackson, R., Munkhtsog, B., Murphy, W. J. (2014). Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples. Conservation Genetic Resource, 6(2), 369:373.
Abstract: Molecular markers that can effectively identify noninvasively collected samples and provide genetic
information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many
noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the
domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard
populations. Combined with seven other loci that we previously described, they have sufficient variation (He = 0.504, An = 3.6) for individual identification and
population structure analysis. We designed a species species specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers
facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species.
endangered species
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