Abstract: Noninvasive fecal assays were used to study steroid metabolism and ovarian activity in several felid species. Using the domestic cat (Felis catus) as model, the excretory products of injected [14C]estradiol (E2) and [14C]progesterone (P4) were determined. Within 2 days, 97.0 +/- 0.6% and 96.7 +/- 0.5% of recovered E2 and P4 radioactivity, respectively, was found in feces. E2 was excreted as unconjugated estradiol and estrone (40%) and as a non-enzyme- hydrolyzable conjugate (60%). P4 was excreted primarily as non-enzyme- hydrolyzable, conjugated metabolites (78%) and as unconjugated pregnenolone epimers. A simple method for extracting fecal steroid metabolites optimized extraction efficiencies of the E2 and P4 excretion products (90.1 +/- 0.8% and 87.2 +/- 1.4%, respectively). Analysis of HPLC fractions of extracted fecal samples from the radiolabel-injected domestic cats revealed that E2 immunoreactivity coincided primarily with the unconjugated metabolized [14C]E2 peak, whereas progestogen immunoreactivity coincided with a single conjugated epimer and multiple unconjugated pregnenolone epimers. After HPLC separation, similar immunoreactive E2 and P4 metabolite profiles were observed in the leopard cat (F. bengalensis), cheetah (Acinonyx jubatus), clouded leopard (Neofelis nebulosa), and snow leopard (Panthera uncia). Longitudinal analyses demonstrated that changes in fecal E2 and P4 metabolite concentrations reflected natural or artificially induced ovarian activity. For example, severalfold increases in E2 excretion were associated with overt estrus or exogenous gonadotropin treatment, and elevated fecal P4 metabolite concentrations occurred during pregnant and nonpregnant (pseudopregnant) luteal phases. Although overall concentrations were similar, the duration of elevated fecal P4 metabolites during pseudopregnancy was approximately half that observed during pregnancy. In summary, steroid metabolism mechanisms appear to be conserved among these physically diverse, taxonomically related species. Results indicate that this hormone-monitoring approach will be extremely useful for elucidating the hormonal regulatory mechanism associated with the reproductive cycle, pregnancy, and parturition of intractable and endangered felid species.

Abstract: In this study, we investigated the cross-reactivity pattern of IgE and IgG4 antibodies to the major feline allergen, Fel d I. We studied the IgE and IgG4 response of 11 cat-allergic patients against Fel d I-like structures in eight members of the Felidae family: ocelot, puma, serval, siberian tiger, lion, jaguar, snow leopard, and caracal. Hair from these “big cats” was collected, extracted, and used in a RAST system and histamine-release test. By means of a RAST-inhibition assay with affinity-purified Fel d I from cat dander, it was established that, in the Felidae species, a Fel d I equivalent is present that reacts with IgE and IgG4 antibodies. We found that all patients had cross-reacting IgE antibodies to seven of the Felidae tested; no IgE antibodies reactive with the caracal were found. Eight of 10 patients with IgG4 antibodies directed to cat dander also had IgG4 antibodies directed to several Felidae species, including the caracal. However, the correlation between the IgE and the IgG4 antibody specificity was low, indicating that, in the case of Fel d I IgE and IgG4, antibodies do not necessarily have the same specificity.

Abstract: In accordance with order of the Ministry of Nature and Tourism,
zoologists of our association have made surveys in three ways such as
reasons why snow leopards attack domestic animals, “Snow leopard” trial
operation to count them and illegal hunting in territories of Khovd,
Gobi-Altai, Bayankhongor, Uvurkhangai and Umnugobi provinces from
September 2009 to January 2010. As result of these surveys it has made
the following conclusions in the followings: Reason to hunt them illegally: the principal reason is that
administrative units have been increased and territories of
administrative units have been diminished. There have been four
provinces in 1924 to 1926, 18 since 1965, 21 since 1990. Such situation
limits movements of herdsmen completely and pastures digressed much than
ever before. As result of such situation, 70% of pastures become desert.
Such digression caused not only heads of animals and also number of
species. Guarantee is that birds such as owls, cuckoo, willow grouse in
banks of Uyert river, Burkhanbuudai mountain, located in Biger soum,
Gobi-Altai province, which are not hunted by hunters, are disappearing
in the recent two decades. For that reason we consider it is urgently
necessary for the government to convert administrative unit structures
into four provinces. This would influence herdsmen moving across
hundreds km and pastures could depart from digression.
Second reason: cooperative movement won. The issues related to management and strengthening of national
cooperatives, considered by Central Committee of Mongolian People's
Revolutionary Party in the meeting in March 1953 was the start of
cooperatives' movement. Consideration by Yu. Tsedenbal, chairman of
Ministers Council, chairman of the MPRP, on report "Result of to unify
popular units and some important issues to maintain entity management of
agricultural cooperatives" in the fourth meeting by the Central
Committee of Mongolian People's Revolutionary Party /MPRP/ on December
16-17, 1959, proclaimed complete victory of cooperative. At the end of
1959, it could unify 767 small cooperative into 389 ones, unify 99.3 %
of herdsmen and socialize 73.3 % of animals. The remaining of animals
amount 6 million 163 thousands animals, and equals to 26.7% of total
animals. This concerned number of animals related to the article
mentioned that every family should have not more that 50 animals in
Khangai zone and not more 75 animals in Gobi desert. It shows that such
number could not satisfy needs of family if such number is divided into
five main animals in separating with reproduction animals and adult
animals. So herdsmen started hunt hoofed animals secretly and illegally
in order to satisfy their meat needs. Those animals included main food
of snow leopard such as ibex, wild sheep, and marmot. Third reason is that the state used to hunt ibex, which are main
nutrition of snow leopards, every year. The administrative unit of the
soum pursued policy to hunt ibex in order to provide meat needs of
secondary schools and hospitals. That's why this affected decrease of
ibex population. Preciously from 1986 to 1990 the permissions to hunt
one thousands of wild sheep and two thousands of ibexes were hunt for
domestic alimentary use every year. Not less than 10 local hunters of every soum used to take part in big
game of ibexes. Also they hunted many ibexes, chose 3-10 best ibexes and
hid them in the mountains for their consummation during hunting.
Fourth reason: hunting of wolves. Until 1990 the state used to give
prizes to hunter, who killed a wolf in any seasons of the year. Firstly
it offered a sheep for the wolf hunter and later it gave 25 tugrugs /15
USD/. Every year, wolf hunting was organized several times especially
picking wolf-cubs influenced spread and population of wolves. So snow
leopard came to the places where wolves survived before and attack
domestic animals. Such situation continued until 1990. Now population of
ibexes has decreased than before 1990 since the state stopped hunting
wolves, population of wolves increased in mountainous zones. We didn't
consider it had been right since it was natural event. However
population of ibexes decreased. Fifth reason: Global warming. In recent five years it has had a drought
and natural disaster from excessive snow in the places where it has
never had such natural disasters before. But Mongolia has 40 million
heads of domestic animals it has never increased like such quantity in
its history before. We consider it is not incorrect that decrease of
domestic animals could give opportunities to raise population of wild
animals. Our next survey is to make attempt to fix heads of snow leopards
correctly with low costs.

Abstract: Ovaries of the seasonally-breeding snow leopard (Uncia uncia) were examined to determine whether they were responsive to exogenous gonadotrophins throughout the year. The potential of laparoscopic artificial insemination (AI) also was assessed for producing offspring. During the non-breeding, pre-breeding, breeding and post-breeding seasons, females (n = 20) were treated with a standardized, dual- hormone regimen given intramuscularly (600 I.U. of equine chorionic gonadotrophin followed 80-84 h later with 300 I.U. of human chorionic gonadotrophin (hCG)). Laparoscopy was performed 45-50 h after administration of hCG, and all ovarian structures were described. Females with fresh corpora lutea (CL) were inseminated, and anovulatory females were subjected to follicular aspiration to examine oocyte quality. Snow leopards responded to exogenous gonadotrophins throughout the year. Mean number of total ovarian structures (distinct follicles mature in appearance plus CL) did not differ (P > or = 0.05) with season, but the proportion of CL: total ovarian structures was greater (P < 0.01) for the breeding season compared with all other seasons. The proportion of females ovulating was greater (P < 0.05) during the breeding and post-breeding seasons than during the pre-breeding and non- breeding seasons respectively. No Grade-1 quality oocytes were recovered from follicles of anovulatory females. Serum concentrations of oestradiol-17 beta appeared elevated in all females, and neither oestradiol-17 beta concentrations nor progesterone concentrations differed (P > or = 0.05) among seasons. Of 15 females artificially inseminated, the only one that was inseminated in the non-breeding season became pregnant and delivered a single cub. This is the first successful pregnancy resulting from AI in this endangered species.

Abstract: Serum oestradiol and progesterone concentrations were measured at weekly intervals for six months, and correlated with daily behavioural observations in two adult female snow leopards (Panthera uncia). Three oestradiol peaks (> 21 pg ml-1; interval 3.6 weeks) were identified in a snow leopardess housed alone (two more were probably missed because of the weekly sampling schedule), and three oestradiol peaks were identified in a snow leopardess housed with a male as a breeding pair (interval 6 weeks). Daily frequencies of feline reproductive behaviour averaged 1.77 observations per observation period during weeks of high oestradiol and 0.62 during weeks of low oestradiol. Progesterone concentrations did not rise above baseline values (< 2 ng ml-1) in the isolated animal, but 6 weeks of high progesterone concentrations (4.9- 38.8 ng ml-1) was recorded in the paired snow leopardess following mating. No offspring were produced. Snow leopards were observed daily for an additional 4.5 years. Sexual behaviour peaks could be clearly identified from December through April, and average daily sexual behaviour scores were higher during these months than during the rest of the year. Intervals between sexual behaviour peaks for the isolated snow leopardess averaged 3.03 weeks. The sexual behaviour of the paired snow leopards decreased for 8-9 weeks following mating when no offspring were produced, and decreased for 13 weeks in one year when a single cub was born.