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Author (up) Janecka, J. E., Jackson, R., Munkhtsog, B., Murphy, W. J. url  openurl
  Title Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples Type Journal Article
  Year 2014 Publication Conservation Genetic Resource Abbreviated Journal  
  Volume 6 Issue 2 Pages 369:373  
  Keywords Microsatellites,Cytochrome b, Snow Leopard, Noninvasive genetics, Individual identification  
  Abstract Molecular markers that can effectively identify noninvasively collected samples and provide genetic

information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many

noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the

domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard

populations. Combined with seven other loci that we previously described, they have sufficient variation (He = 0.504, An = 3.6) for individual identification and

population structure analysis. We designed a species species specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers

facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species.

endangered species
 
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  ISSN ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number SLN @ rakhee @ Serial 1427  
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