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Author |
Waits, L.P.; Buckley-Beason, V.A.; Johnson, W.E.; Onorato, D.; McCarthy, T. |
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Title |
A select panel of polymorphic microsatellite loci for individual identification of snow leopards (Panthera uncia)
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Miscellaneous |
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2006 |
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Molecular Ecology Notes |
Abbreviated Journal |
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7 |
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311-314 |
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Keywords |
identification; leopard; leopards; microsatellites,noninvasive genetic sampling,Panthera uncia,snow leopard; panthera; Panthera-uncia; panthera uncia; snow; snow-leopard; snow-leopards; snow leopard; snow leopards; uncia; endangered; carnivores; carnivore; mountain; region; central; Central Asia; asia; methods; conservation; management; Molecular; individual identification; Hair; domestic; cat; felis; captive; number; probability; using; wild |
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Snow leopards (Panthera uncia) are elusive endangered carnivores found in remote mountain regions of Central Asia. New methods for identifying and counting snow leopards are needed for conservation and management efforts. To develop molecular genetic tools for individual identification of hair and faecal samples, we screened 50 microsatellite loci developed for the domestic cat (Felis catus) in 19 captive snow leopards. Forty-eight loci were polymorphic with numbers of alleles per locus ranging from two to 11. The probability of observing matching genotypes for unrelated individuals (2.1 x10-11) and siblings (7.5x10-5) using the 10 most polymorphic loci was low, suggesting that this panel would easily discriminate among individuals in the wild. |
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SLN @ rana @ 865 |
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1001 |
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Author |
Zhang, F.; Jiang, Z.; Zeng, Y.; McCarthy, T. |
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Title |
Development of primers to characterize the mitochondrial control region of the snow leopard (Uncia uncia) |
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Miscellaneous |
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Year |
2007 |
Publication |
Molecular Ecology Notes |
Abbreviated Journal |
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7 |
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1196-1198 |
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Keywords |
control region,Felidae,mitochondrial DNA,snow leopard,species-specific primers; genetics; development; mitochondrial; control; region; snow; snow leopard; snow-leopard; leopard; uncia |
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Abstract |
The snow leopard (Uncia uncia) is a rare carnivore living above the snow line in central Asia. Using universal primers for the mitochondrial genome control region hypervariable
region 1 (HVR1), we isolated a 411-bp fragment of HVR1 and then designed specific primers
near each end of this sequence in the conserved regions. These primers were shown to yield
good polymerase chain reaction products and to be species specific. Of the 12 snow leopards
studied, there were 11 segregating sites and six haplotypes. An identification case of snow
leopard carcass (confiscated by the police) proved the primers to be a useful tool for forensic
diagnosis in field and population genetics studies. |
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SLN @ rana @ 911 |
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1073 |
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