Records |
Author |
Janeč ka, J.E., Munkhtsog, B., Jackson, R.M., Naranbaatar, G., Mallon, D.P. & Murphy, W.J. |
Title |
Comparison of noninvasive genetic and camera-trapping techniques for surveying snow leopards |
Type |
Journal Article |
Year |
2011 |
Publication |
Journal of Mammalogy |
Abbreviated Journal |
|
Volume |
92 |
Issue |
4 |
Pages |
771-783 |
Keywords |
|
Abstract |
The endangered snow leopard (Panthera uncia) is widely but sparsely distributed throughout the mountainous regions of central Asia. Detailed information on the status and abundance of the snow leopard is limited because of the logistical challenges faced when working in the rugged terrain it occupies, along with its secretive nature. Camera-trapping and noninvasive genetic techniques have been used successfully to survey this felid. We compared noninvasive genetic and camera-trapping snow leopard surveys in the Gobi Desert of Mongolia. We collected 180 putative snow leopard scats from 3 sites during an 8-day period along 37.74 km of transects. We then conducted a 65-day photographic survey at 1 of these sites, approximately 2 months after scat collection. In the site where both techniques were used noninvasive genetics detected 5 individuals in only 2 days of fieldwork compared to 7 individuals observed in the 65-day camera-trapping session. Estimates of population size from noninvasive genetics ranged between 16 and 19 snow leopards in the 314.3-km2 area surveyed, yielding densities of 4.9–5.9 individuals/100 km2. In comparison, the population estimate from the 65-day photographic survey was 4 individuals (adults only) within the 264-km2 area, for a density estimate of 1.5 snow leopards/100 km2. Higher density estimates from the noninvasive genetic survey were due partly to an inability to determine age and exclude subadults, reduced spatial distribution of sampling points as a consequence of collecting scats along linear transects, and deposition of scats by multiple snow leopards on common sites. Resulting differences could inflate abundance estimated from noninvasive genetic surveys and prevent direct comparison of densities derived from the 2 approaches unless appropriate adjustments are made to the study design. |
Address |
|
Corporate Author |
|
Thesis |
|
Publisher |
American Society of Mammalogists |
Place of Publication |
|
Editor |
|
Language |
English |
Summary Language |
|
Original Title |
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
Series Volume |
|
Series Issue |
|
Edition |
|
ISSN |
|
ISBN |
|
Medium |
|
Area |
|
Expedition |
|
Conference |
|
Notes |
DOI: 10.1644/10-MAMM-A-036.1; URL: http://www.bioone.org/doi/full/10.1644/10-MAMM-A-036.1 |
Approved |
no |
Call Number |
SLN @ rana @ |
Serial |
1351 |
Permanent link to this record |
|
|
|
Author |
Janecka; J.E.; Jackson, R.; Yuquang, Z.; Diqiang, L.; Munkhtsog, B.; Buckley-Beason, V.; Murphy, W.J. |
Title |
Population monitoring of snow leopards using noninvasive collection of scat samples: a pilot study |
Type |
Miscellaneous |
Year |
2008 |
Publication |
Animal Conservation |
Abbreviated Journal |
|
Volume |
11 |
Issue |
|
Pages |
401-411 |
Keywords |
snow leopard; genetics; scat; noninvasive; survey. |
Abstract |
The endangered snow leopard Panthera uncia occurs in rugged, high-altitude regions of Central Asia. However, information on the status of this felid is limited in many areas. We conducted a pilot study to optimize molecular markers for the analysis of snow leopard scat samples and to examine the feasibility of using noninvasive genetic methods for monitoring this felid. We designed snow leopard-specific primers for seven microsatellite loci that amplified shorter segments and avoided flanking sequences shared with repetitive elements. By redesigning primers we maximized genotyping success and minimized genotyping errors. In addition, we tested a Y chromosome-marker for sex identification and designed a panel of mitochondrial DNA primers for examining genetic diversity of snow leopards using scat samples. We collected scats believed to be from snow leopards in three separate geographic regions including north-western India, central China and southern Mongolia. We observed snow leopard scats in all three sites despite only brief 2-day surveys in each area. There was a high rate of species misidentification in the field with up to 54% of snow leopard scats misidentified as red fox. The high rate of field misidentification suggests sign surveys incorporating scat likely overestimate snow leopard abundance. The highest ratio of snow leopard scats was observed in Ladakh (India) and South Gobi (Mongolia), where four and five snow leopards were detected, respectively. Our findings describe a species-specific molecular panel for analysis of snow leopard scats, and highlight the efficacy of noninvasive genetic surveys for monitoring snow leopards. These methods enable large-scale noninvasive studies that will provide information critical for conservation of snow leopards. |
Address |
|
Corporate Author |
|
Thesis |
|
Publisher |
|
Place of Publication |
|
Editor |
|
Language |
|
Summary Language |
|
Original Title |
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
Series Volume |
|
Series Issue |
|
Edition |
|
ISSN |
|
ISBN |
|
Medium |
|
Area |
|
Expedition |
|
Conference |
|
Notes |
Supplemental information may be found at http:snowleopardnetwork.org/bibliography/JaneckaSupplemental2008.pdf |
Approved |
no |
Call Number |
SLN @ rana @ 883 |
Serial |
488 |
Permanent link to this record |
|
|
|
Author |
Johnson, W.E.; Eizirik, E.; Pecon-Slattery, J.; Murphy, W.J.; Antunes, A.; Teeling, E.; O'Brien, S.J. |
Title |
The Late Miocene Radiation of Modern Felidae: A Genetic Assessment |
Type |
Miscellaneous |
Year |
2006 |
Publication |
Science |
Abbreviated Journal |
|
Volume |
311 |
Issue |
|
Pages |
74-77 |
Keywords |
carnivore; classification; divergence; Dna; Felidae; fossil; lineages; Miocene; mitochondrial; Molecular; phylogeny; radiation; taxonomic |
Abstract |
Modern felid species descend from relatively recent (G11 million years ago) divergence and speciation events that produced successful predatory carnivores worldwide but that have confounded taxonomic classifications. A highly resolved molecular phylogeny with divergence dates for all living cat species, derived from autosomal, X-linked, Y-linked, and mitochondrial gene segments (22,789 base pairs) and 16 fossil calibrations define eight principal lineages produced through at least 10 intercontinental migrations facilitated by sea-level fluctuations. A ghost lineage analysis indicates that available felid fossils underestimate (i.e., unrepresented basal branch length) first occurrence by an average of 76%, revealing a low representation of felid lineages in paleontological remains. The phylogenetic performance of distinct gene classes showed that Y-chromosome segments are appreciably more informative than mitochondrial DNA, X-linked, or autosomal genes in resolving the rapid Felidae species radiation. |
Address |
|
Corporate Author |
|
Thesis |
|
Publisher |
|
Place of Publication |
|
Editor |
|
Language |
|
Summary Language |
|
Original Title |
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
Series Volume |
|
Series Issue |
|
Edition |
|
ISSN |
|
ISBN |
|
Medium |
|
Area |
|
Expedition |
|
Conference |
|
Notes |
|
Approved |
no |
Call Number |
SLN @ rana @ 908 |
Serial |
502 |
Permanent link to this record |